Molecular Phenotyping

Equipment & Services Available

The Molecular Phenotyping core provides instrumentation, training, and services for in vitro cell phenotyping.

biomek-3000_600p

BIOMEK 3000: Automated liquid handling.

biorad-cfx384_600p

BIORAD CFX CONNECT, CFX 384, MyIQ and iQ5: Real-time PCR (SYBR and TaqMan)

diagenode_straight_278p

DIAGENODE BIORUPTOR 300: Chromatin shearing for chromatin immunoprecipitation (ChIP), genomic DNA shearing for next generation sequencing, RNA shearing, cell and tissue disruption, FFPE DNA extraction. [Learn More]

fluidigm_340p

FLUIDIGM C1: Single cell isolation for mRNA sequencing, qPCR, DNA sequencing, epigenetics or miRNA expression.  [Learn More]

leica-dm-irb_600

LEICA DM IRB microscope: 4 color inverted epifluorescence microscopy. [Learn More]

leica-dmi6000b_600

LEICA DMI6000B fitted with BL-X culture chamber: Fully automated 4 color epifluorescence live-cell microscopy

nanodrop_340p

NANODROP 2000: UV-Vis spectrophotometry

qiacube_340

QIAcube: Fully automated sample prep using QIAGEN spin-column kits (DNA, RNA, or protein)

Service Cost
Fluidigm C1: C1 Cell Prep Contact Michele Karolak for pricing details
Fluidigm C1: Preamp for DeltaGene Contact Michele Karolak for pricing details
Fluidigm C1: Preamp for TaqMan Contact Michele Karolak for pricing details
Fluidigm C1: Library for mRNA seq Contact Michele Karolak for pricing details
BIORAD CFX Connect, CFX 384, MyIQ, and iQ5 $3.50/hour
Biomek 3000 (not including consumables) $10/run
Nanodrop 2000 $2/run
Leica DM IRB (Inverted epifluorescence microscopy) $5/hour
Leica DMI 6000B (Epifluorescence live cell microscopy) $10/hour
QIAcube: RNeasy Micro $10.82/sample
QIAcube: RNeay Mini $6.96/sample
QIAcube: DNeasy Blood and Tissue $4.05/sample
QIAcube:QIAprep Spin Miniprep $2.69/sample
Diagenode Bioruptor $5/run
Tissue Homogenizer $20/50g Zirconium beads

Contact Us

Director: Leif Oxburgh, DVM, PhD
oxburl@mmc.org

Manager: Michele Karolak
karolm@mmc.org

Request Training

Users must be appropriately trained before using core equipment.

Instrument training: contact Michele Karolak.
Workstation training: contact Dr. Oxburgh