The Physiology Core is focused on assessing physiological and metabolic phenotypes for novel mouse models of human disease. To complement assays for biochemical markers of skeletal and soft tissue metabolism, Promethion metabolic cages provide 87 distinct readings per second on mouse activity, oxygen consumption, CO2 production, food intake, weight and water consumption. This output is coupled with dual energy X-ray absorptiometry/NMR analysis that measures in vivo body composition cross-sectionally or longitudinally.
A Seahorse XF96 analyzer that measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis is also available. The XF96 provides bioenergetic data utilizing three agents in a mitochondrial or glycolytic rate test paradigm. In addition a comprehensive metabolic report is provided by the Core director to the investigator that emphasizes the metabolic phenotype of the animal, alternative experiments or biomarkers to be considered, and suggestions for further studies.
- Body Composition/ Metabolic cage Analysis
- Insulin/Glucose Tolerance Test
- Immunoassays for Biomarkers
- Celullar Bioenergetic Analysis
- Data Analysis and Interpretation
- Promethion metabolic monitoring cage system
- PIXImus densitomer
- Bruker minispec mq NMR
- Seahorse XF96 extracellular flux analyzer
- Idexx ProCyte Dx hematology analyzer
|Promethion Metabolic Cages (includes NMR scan)
|Seahorse BioScience XF24