Primary research project focused on mechanotransduction of bone, specifically, how Sirtuin1 could regulate bone metabolism during increased loading or disuse. The implications of this work were targeted towards populations that experience disuse osteopenia (i.e., astronauts and bedridden patients). Both in vivo (mouse models of hindlimb suspension and tibial compression) and in vitro (oscillatory fluid flow) experiments were carried out to investigate this relationship. Other research projects included the screening of Alk2 inhibitors for the treatment of fibrodysplasia ossificans progressiva (FOP) and the role of Tgfbr3 in bone metabolism.
Doctoral: Ph.D. thesis focused on (1) mouse models of diet induced obesity (C57BL/6J, C57BL/6N, C3H/HeJ) and examining alterations in bone metabolism, microarchitecture, and biomechanical; (2) investigating the role of toll-like receptor (TLR)-4 in this skeletal response; and (3) exploring the extent to which the autophagic pathway in bone cells is altered in response to impaired insulin signaling and glucose availability. The results from these studies (1) established an appropriate mouse model for the pathogenesis of T2DM; (2) suggested that the metabolic changes associated with a high fat diet attenuate bone accrual by altering bone turnover, and that TLR-4 is involved in this skeletal phenotype; and (3) Beclin1-mediated autophagy appears to be up-regulated during impaired glucose tolerance, driving the osteoblast towards a more mature, non-mineralizing phenotype.
M.S.: Master’s thesis was focused on bioactive foods, specifically dried plum, and their implications on bone microacrhitecture, osteoblasts, and osteoclasts.
Jackson EE, Rendina-Ruedy E
, Smith BJ, Lacombe VA (2015) Loss of Toll-Like Receptor 4 Function Partially Protects against Peripheral and Cardiac Glucose Metabolic Derangements During a Long-Term High-Fat Diet. PLoS ONE 10(11): e0142077.
E Rendina-Ruedy, JL Graef, M.R. Davis, KD Hembree, JM Gimble, SL Clarke, EA Lucas, BJ Smith. Strain Differences in the Attenuation of Bone Accrual in a Young Growing Mouse Model of Insulin Resistance. J Bone Miner Metab. 2015 Jun 10. [Epub ahead of print]. PMID: 26058493.
Rendina-Ruedy, K. D. Hembree, A. Sasaki, M. R. Davis, S. L. Clarke, E. A. Lucas, B.J. Smith. A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes. J Nutr Metab. 2015;2015:758080. doi: 10.1155/2015/758080. Epub 2015 Jun 3. PMID: 26146567.
Amanda J. Taylor, Elizabeth Rendina, Brenda J. Smith, Donghua H. Zhou. Analyses of mineral specific surface area and hydroxyl substitution for intact bone. Chem Phys Lett. 2013 Nov 19; 588. doi:10.1016/j.cplett.2013.09.061. PMID: 24347673.
Smith BJ, Bu SY, Wang Y, Rendina E, Lim YF, Marlow D, Clarke SL, Cullen DM, Lucas EA. A Comparative Study of the Bone Metabolic Response to Dried Plum Supplementation and PTH Treatment in Adult, Osteopenic Ovariectomized Rat Bone.Bone. 2014 Jan;58:151-9. doi: 10.1016/j.bone.2013.10.005. Epub 2013 Oct 11. PMID:24125756
Smith BJ, Graef JL, Rendina E, Clark KA, Clarke SL, Lucas EA, Wronski TJ, Halloran BP. Effects of Dried Plum Supplementation on Bone Metabolism in Adult C57BL/6 Male Mice. Calcif Tissue Int. 2014 Apr;94(4):442-53. doi: 10.1007/s00223-013-9819-2. Epub 2013 Dec 20. PMID:24357047.
Rendina, K. D. Hembree, M.R. Davis, D. Marlow, S. L. Clarke, B.P Halloran, E. A. Lucas, B. J. Smith. Dried Plum’s Unique Capacity to Reverse Bone Loss and Alter Bone Metabolism in a Postmenopausal Osteoporosis Model. PLoS ONE 8(3): e60569. doi:10.1371/journal.pone.0060569. PMID:23555991
R. Davis, E. Rendina, S.K. Peterson, E.A. Lucas, B.J. Smith, and S.L. Clarke. Enhanced expression of lipogenic genes may contribute to hyperglycemia and alterations in plasma lipids in response to dietary iron deficiency. Genes Nutr. 2012; 7(3): 415-23. doi: 10.1007/s12263-011-0278-y. Epub 2012 Jan 7. PMID:22228222
Rendina, Y. F. Lim, D. Marlow, Y. Wang, S. L. Clarke, S. Kuvibidila, E. A. Lucas, B. J. Smith. Dietary Supplementation with Dried Plum Prevents Ovariectomy-Induced Bone Loss While Modulating the Immune Response in C57BL/6J Mice. Journal of Nutritional Biochemistry 2012; 23 (60-68). doi: 10.1016/j.jnutbio.2010.10.010. Epub 2011 Mar 16. PMID: 21414765
R. Davis, K.M. Shawron, S.K. Peterson, E.Rendina, E.A. Lucas, B.J. Smith, and S.L. Clarke. Hypoxia Inducible Factor 2-alpha is Translationally Repressed in Response to a Dietary Iron Deficiency in Sprague-Dawley Rats. Journal of Nutrition 2011 Sept;141(9):1590-6. doi: 10.3945/jn.111.144105. Epub 2011 Jul 13. PMID: 21753061