Pradeep Sathyanarayana, PhD

Pradeep Sathyanarayana, PhD 2017-08-08T12:10:08+00:00

Pradeep Sathyanarayana, PhD

Faculty Scientist I

EDUCATION

MS: Biochemistry, University of Mysore, India
PhD: Biochemistry, Indian Institute of Toxicological Research, India
Postdoctoral Training: Massachusetts General Hospital (Harvard Medical School),Boston, MA & Cardiovascular Research Institute, Texas A & M University Health Science Center, Temple, TX

Once hidden in the ‘dark genomic matter’, microRNAs (miRNAs) are emerging as important factors in the pathophysiology of cancer, including leukemias. Upregulation of miRNAs that target tumor suppressors or downregulation of miRNAs that target oncogenes can result in tumorigenesis. Such mechanisms may be relevant in leukemia. Thus, specific miRNA expression profiles have been associated with distinct genetic abnormalities in AML, supporting a mechanistic involvement. However, the identity and functions of miRNAs in the initiation and development of leukemia remain largely unknown. Investigations in our lab are largely focused on a detailed understanding of miRNAs role in leukemogenesis, which needs to be established in order to fully explore their clinical translational potential. Our mechanistic studies will inform novel and exciting therapeutic opportunities in AML.

Role of Oncogenic microRNAs in Myelopoiseis and Myeloid Leukemia

Sathyanarayana_Fig_1Hematopoiesis, the process by which hematopoietic stem cells (HSC) either self-renew or commit to differentiation into discrete lineages, is tightly regulated by both intrinsic factors (signaling networks and transcription factors) and extrinsic factors (bone marrow microenvironment, cell adhesion molecules and cytokines. In general, cytokines regulate hematopoiesis by triggering one or more signaling cascades, with IL-3, GM-CSF and G-CSF acting as dominant regulators of the growth and differentiation of myeloid progenitors. In myeloid progenitor cells, they signal via a common JAK2-STAT5 pathway. MicroRNAs add another regulatory dimension to hematopoiesis, by selectively targeting signaling molecules, receptors, ligands and transcription factors, thereby influencing functional outcomes related to HSC self-renewal, differentiation, apoptosis and the balance of myeloid vs. lymphoid populations within the hematopoietic progenitor pool.

Role of Tumor Suppressor microRNAs in Myeloid Leukemia

Studies in our lab are also focused on two miroRNAs miR-199b and miR-125a that are significantly silenced in AML. Though we and others have previously demonstrated their respective silencing in AML, their clinical significance, mechanism of action, and therapeutic potential is yet to be deciphered. For miR-199b, our ongoing studies have identified novel cooperating mutations that associate significant correlation with low miR-199b in AML and significantly affect the survival outcomes. Equally exciting are the outcomes from miR-199b knockout mouse model. For miR-125a, after defining its molecular basis of its epigenetic dysregulation and identifying potential targets via profiling studies in gain-of-function model, our ongoing studies are focused on determining its clinical significance and its action mechanism.

Novel Roles of Podocalyxin in Myelopoiesis and Myeloid Leukemia

Podocalyxin (PodxL) is a CD34 family member previously identified to mark hematopoietic stem cells (HSCs) and other progenitor cells. Previously, we discovered PodxL as a potent Erythropoietin (EPO) response gene and demonstrated to promote egression of immature reticulocytes from bone marrow into circulation. PodxL is upregulated in several cancers, including myeloid and lymphoid leukemia. Our lab discovered PodxL as a bona fide target of miR-199b. Current investigations aim to define the functional role of PodxL in hematopoiesis – specifically myelopoiesis – by employing conditional PodxL knock out (KO) mouse models where hematopoietic-specific deletion via Vav1-Cre driver and myeloid-specific deletion via Lyzm2 – Cre driver are being utilized.

Aldona Karaczyn, PhD

Research Fellow
karaca@mmc.org

Research Interests: Learn more about Dr. Karaczyn’s research

 

A complete list of publications can be found on My NCBI

Ufkin ML, Peterson S, Yang X, Driscoll H, Duarte C and Sathyanarayana P.  (2014). miR-125a regulates cell cycle, proliferation, and apoptosis by targeting the ErbB Pathway in Acute Myeloid Leukemia. Leukemia Research. Jan 8. pii: S0145-2126(13)00453-0. doi: 10.1016/j.leukres.2013.12.021. PMCID: PMC4117580.

Favreau AJ, Vary CP, Brooks PC and Sathyanarayana P. (2014). Cryptic collagen IV promotes cell migration and adhesion in myeloid leukemia. Cancer Medicine. Feb 12. doi: 10.1002/cam4.203. PMCID: PMC3987076.

Favreau AJ and Sathyanarayana, P (2012). miR-590-5p, miR-219-5p, miR-15b and miR-628-5p are commonly regulated by IL-3, GM-CSF and G-CSF in acute myeloid leukemia.  Leukemia Research, 36;334– 341. PMCID: PMC3264807.

Favreau AJ, Cross E, Sathyanarayana, P (2012).  miR-199b-5p directly targets PODXL and DDR1 and decreased levels of miR-199b-5p correlate with elevated expressions of PODXL and DDR1 in  Acute Myeloid Leukemia.  American Journal of Hematology.  Apr; 87(4):442-6. PMCID: PMC4104571.

Sathyanarayana P, Dev A, Pradeep A, Ufkin M, Licht JD, Wojchowski DM  (2012) Spry1 as a novel regulator of erythropoiesis, EPO/EPOR target, and suppressor of JAK2.  BLOOD. Jun 7;119:5522-31. PMCID: PMC3369686.

Sathyanarayana P, Houde E, Marshall D, Volk A, Makropoulos D, Emerson C, Pradeep A, Bugelski P, and Wojchowski, D.M. (2009). CNTO-530 acts as a potent EPO mimetic via the sustained expansion of a late erythroid progenitor pool. BLOOD; May 14; 113(20):4955-62. PMCID: PMC2686145.

Sathyanarayana P, Dev A, Fang J, Houde E, Bogacheva O, Bogachev O, Menon M, Browne S, Pradeep A, Emerson C, and Wojchowski DM. (2008). EPO receptor circuits for Primary Erythroblast survival. BLOOD; Jun 1;111(11):5390-9. PMCID: PMC2396729.

Sathyanarayana P, Menon M, Bogacheva O, Bogachev O, Kapelle WS, Houde E, Niss K, Wojchowski DM. (2007) Erythropoietin modulation of Podocalyxin, and an Erythroblast niche. BLOOD; Jul 15;110(2):509-18.

Sathyanarayana P, Barthwal MK, Kundu CN, Lane ME, Bergmann A, Tzivion G, Rana A. (2002). Activation of the Drosophila MLK by ceramide reveals TNF-a and ceramide as agonists of mammalian MLK3. Molecular CELL; 10:1527-1533.

Sathyanarayana P, Barthwal MK, Lane ME, Acevedo SF, Skoulakis EMC, Bergmann A, Rana A. (2003). Drosophila mixed lineage kinase, a missing biochemical link in Drosophila JNK signaling. Biochim Biophys Acta. – Molecular Cell Research; 1640:77-84.

Academic Affiliations

  • Graduate Faculty, School of Biomedical Sciences, University of Maine, Orono, ME
  • Assistant Professor, Department of Medicine, Tufts University School of Medicine, Boston, MA
  • Assistant Professor, Tufts Clinical and Translational Science Institute (CTSI), Boston, MA

Professional Activities

  • Active Member, American Society of Hematology
  • Reviewer, American Heart Association’s (AHA) Peer Review Committee on Molecular Signaling-1 Study Section