Understanding the metabolic and biochemical fate of marrow stromal cells as progenitor osteoblasts and adipocytes.
The central theme of the Rosen Laboratory is understanding the metabolic and biochemical fate of marrow stromal cells as progenitor osteoblasts and/or adipocytes. In vivo, this translates into defining the relationship between marrow adipogenesis and osteoblastogenesis, and the interactions between whole body and skeletal metabolism. We use age, genetic, environmental, diet, and pharmacologic manipulations in order to understand the complex regulation of bone remodeling. We use a variety of techniques to address our research questions including Faxitron imaging, NMR, microCT, MRI, osmium tetroxide staining, histomorphometry, immunohistochemistry, immunofluorescence, confocal microscopy, Seahorse extracellular flux analysis, and metabolic in vivo studies using Promethion technology.
The lab has strong collaborative work with internal and external laboratories and hospitals world-wide involving sophisticated primary culturing of osteoblast, osteoclasts, osteocytes, and adipocytes. We combine our in vivo observations with in vitro cell culture models in order to understand the mechanisms responsible for bone and fat interactions.